Poster Presentation Australian Society for Microbiology Annual Scientific Meeting 2018

Statins promote clearance of Alzheimer’s amyloid beta fused to green fluorescent protein in yeast (#324)

Ian Macreadie 1 , Ken Gardiner 1 , Mishal Subhan 2 , Rani Faryal 2
  1. RMIT University, Bundoora, VIC, Australia
  2. Quaid-i-Azam University, Islamabad, Pakistan

A large-scale epidemiology study on statins previously showed that simvastatin was unique among statins in reducing the incidence of dementia.  Since amyloid beta (Aβ) is the protein that is most associated with Alzheimer’s disease, this study has focused on how three statins, lovastatin, simvastatin and atorvastatin, influence the turnover of Aβ fused with green fluorescent protein (GFP), in the simplest eukaryotic model organism, Saccharomyces cerevisiae.  Flow cytometry was employed to evaluate the proportion of cells having GFP fused to Aβ.  All statins reduced levels of Aβ fused to GFP, however, simvastatin promoted the greatest clearance.  A comparison with fluconazole, which targets that same pathway, suggests that effects of ergosterol synthesis may not account for the clearance of Aβ fused to GFP.  This is the first report of statins being involved in clearance of Aβ fused to GFP, providing new insights into how simvastatin exerts its neuroprotective role.